Essien E E, Afamefuna G C
Clin Chem. 1982 May;28(5):1148-52.
Chloroquine, its N-dealkylated metabolites, and chloroquine N-oxides were detected in the urine of pregnant women who were receiving chloroquine medication whereas chloroquine and its nonpolar metabolites, desethyl- and didesethylchloroquine and 7-chloro-4-aminoquinoline, have been found in the neonates' urine, blood, and cord blood. We used thin-layer chromatography to separate chloroquine and its metabolites after their extraction from biological fluids. These compounds were identified by comparing their chromatographic and ultraviolet spectrophotometric characteristics with those of reference compounds. That chloroquine and its relatively nonpolar metabolites (including one without the alkyl side-chain, 7-chloro-4-aminoquinoline) cross the placenta is demonstrated by the presence of these compounds in the cord blood, neonatal systemic blood, and neonatal urine. The selective transfer of the compounds in the cord blood, neonatal system blood, and neonatal urine. The selective transfer of the compounds across the placenta and the clinical implications of these findings are discussed.
在接受氯喹治疗的孕妇尿液中检测到了氯喹、其N-去烷基代谢产物以及氯喹N-氧化物，而在新生儿的尿液、血液和脐带血中发现了氯喹及其非极性代谢产物，即去乙基氯喹、双去乙基氯喹和7-氯-4-氨基喹啉。我们采用薄层色谱法从生物流体中提取氯喹及其代谢产物后进行分离。通过将这些化合物的色谱和紫外分光光度特征与参考化合物的特征进行比较来进行鉴定。脐带血、新生儿全身血液和新生儿尿液中存在这些化合物，这证明氯喹及其相对非极性的代谢产物（包括一种没有烷基侧链的代谢产物，7-氯-4-氨基喹啉）能够穿过胎盘。讨论了这些化合物在脐带血、新生儿系统血液和新生儿尿液中的选择性转运。还讨论了这些化合物穿过胎盘的选择性转运情况以及这些发现的临床意义。
