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化学研究作为化脓棒状杆菌和“溶血棒状杆菌”分类的指南。

Chemical studies as a guide to the classification of Corynebacterium pyogenes and "Corynebacterium haemolyticum".

作者信息

Collins M D, Jones D, Kroppenstedt R M, Schleifer K H

出版信息

J Gen Microbiol. 1982 Feb;128(2):335-41. doi: 10.1099/00221287-128-2-335.

DOI:10.1099/00221287-128-2-335
PMID:7077293
Abstract

The peptidoglycan of Corynebacterium pyogenes and "Corynebacterium haemolyticum" contained lysine as the dibasic amino acid. In addition, glutamic acid and alanine were detected in the hydrolysates. Thin-layer chromatographic analysis of whole-organism methanolysates of C. pyogenes and "C. haemolyticum" revealed the presence of single spots corresponding to simple non-hydroxylated fatty acid methyl esters. The major fatty acid structural types were straight-chain and monounsaturated acids. Menaquinones (vitamin K) were the sole respiratory quinones detected in C. pyogenes and "C. haemolyticum". The major menaquinone components in C. pyogenes were tetrahydrogenated with ten isoprene units. Tetrahydrogenated menaquinones with nine isoprene units, however, predominated in "C. haemolyticum". The results of the present study indicate that C. pyogenes should be excluded from the genus Corynebacterium: C. pyogenes can be accommodated in the genus Actinomyces but the taxonomic position of "C.haemolyticum " remains equivocal.

摘要

化脓棒状杆菌和“溶血棒状杆菌”的肽聚糖含有赖氨酸作为二氨基氨基酸。此外,在水解产物中检测到谷氨酸和丙氨酸。对化脓棒状杆菌和“溶血棒状杆菌”全菌体甲醇解产物的薄层色谱分析显示,存在与简单非羟基化脂肪酸甲酯相对应的单个斑点。主要脂肪酸结构类型为直链和单不饱和酸。甲萘醌(维生素K)是在化脓棒状杆菌和“溶血棒状杆菌”中检测到的唯一呼吸醌。化脓棒状杆菌中的主要甲萘醌成分是具有十个异戊二烯单元的四氢化甲萘醌。然而,具有九个异戊二烯单元的四氢化甲萘醌在“溶血棒状杆菌”中占主导地位。本研究结果表明,化脓棒状杆菌应从棒状杆菌属中排除:化脓棒状杆菌可归入放线菌属,但“溶血棒状杆菌”的分类地位仍不明确。

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