Barford P A, Staff F J, Blair J A
Biochem J. 1978 Aug 15;174(2):579-83. doi: 10.1042/bj1740579.
The metabolism of [2-14C]folic acid over 13 days and a mixture of [2-14C]- and [3',5',9-3h]-folic acid in rats over a 6-day period is described. Both 14C and 3H are excreted in urine over the 6-day period, but 3H and 14C are only detectable in faeces for 2 days. A breakdown product of folic acid labelled with 3H only was found in some urine samples, but no metabolite corresponding to the part of the molecule containing 14C was detected. These experiments show that in the whole animal a substantial portion of orally administered folic acid undergoes scission shortly after administration [Blair Biochem. J. (1957) 68, 385-387] and that the retained folates are a shortage form for folate monoglutamates.
描述了[2-¹⁴C]叶酸在大鼠体内13天的代谢情况以及[2-¹⁴C]-和[3',5',9-³H]-叶酸混合物在大鼠体内6天的代谢情况。在这6天期间，¹⁴C和³H均通过尿液排出，但³H和¹⁴C仅在粪便中可检测到2天。在一些尿液样本中发现了仅用³H标记的叶酸分解产物，但未检测到与含有¹⁴C的分子部分相对应的代谢物。这些实验表明，在完整动物中，口服叶酸的很大一部分在给药后不久就会发生断裂[布莱尔，《生物化学杂志》（1957年）68卷，385 - 387页]，并且保留的叶酸是叶酸单谷氨酸的短缺形式。
