Erbrich U, Naujok A, Petschel K, Zimmermann H W
Histochemistry. 1982;74(1):1-7. doi: 10.1007/BF00495046.
The fluorescent staining of mitochondria in living cells with new acridine dyes is reported. The fluorescent dyes used are derivatives of acridine orange (AO) and of 3-amino-6-methoxyacridine (AMA) with various residues in 9- or 10-position (Scheme 1). They are either permanent cationic dyes or cations which are formed by protonation in the culture medium. HeLa cells and mouse fibroblasts (LM cells) have been used for our staining experiments. On favourable conditions we succeeded in staining the mitochondria not only orthochromatically but also metachromatically. Photodynamical effects which have been observed during the exposure of the stained cells in the fluorescence microscope are described. The residues in 9- or 10-position favour the dye accumulation in the mitochondria. Vital staining with the basic compounds AO and AMA however leads to the formation of metachromatically stained lysosomes in the orthochromatically stained cytoplasm. The dye 3-amino-6-methoxy-9-(2-hydroxyethyl)acridine stains the nucleus of living cells.
报道了用新型吖啶染料对活细胞中线粒体进行荧光染色的情况。所用的荧光染料是吖啶橙（AO）和3-氨基-6-甲氧基吖啶（AMA）的衍生物，在9位或10位带有各种取代基（方案1）。它们要么是永久性阳离子染料，要么是在培养基中通过质子化形成的阳离子。我们使用了HeLa细胞和小鼠成纤维细胞（LM细胞）进行染色实验。在有利条件下，我们成功地不仅以正色方式而且以异染方式对线粒体进行了染色。描述了在荧光显微镜下对染色细胞进行照射期间观察到的光动力效应。9位或10位的取代基有利于染料在线粒体中的积累。然而，用碱性化合物AO和AMA进行活体染色会导致在正色染色的细胞质中形成异染染色的溶酶体。染料3-氨基-6-甲氧基-9-（2-羟乙基）吖啶可对活细胞的细胞核进行染色。
