Witkin S S, Zelikovsky G, Bongiovanni A M, Geller N, Good R A, Day N K
J Clin Invest. 1982 Jul;70(1):33-40. doi: 10.1172/jci110600.
Sera from 35 men were collected before and at timed intervals subsequent to vasectomy and examined for the presence of (a) antibody reactive with human spermatozoa, (b) sperm-related antigen, and (c) circulating immune complexes (CIC). Fewer than 10% of the men examined were ever positive for antisperm antibodies. However, sperm-related antigens were elevated in the sera of 18, 18, and 26% of the mean at 2 wk, 2 mo, and 4 mo postvasectomy, respectively. CIC were detected in the sera of some vasectomized men by three different assays. The CIC in patients' sera were precipitated with polyethylene glycol, dissociated, and the individual CIC components identified by an enzyme-linked immunosorbent assay. Most, but not all, of the CIC contained antigen reactive with antisperm immunoglobulin (Ig)G and some also contained complement components C3 and/or Clq. IgA was identified in some of the CIC positive for IgG and sperm antigen and two men had IgM-containing CIC. Analysis of the CIC by sucrose gradient centrifugation revealed them to be heterogeneous in size.
采集了35名男性输精管切除术前及术后不同时间间隔的血清，检测其中是否存在：(a) 与人精子发生反应的抗体，(b) 精子相关抗原，以及 (c) 循环免疫复合物（CIC）。接受检测的男性中，抗精子抗体呈阳性的比例不到10%。然而，在输精管切除术后2周、2个月和4个月时，分别有18%、18%和26%的男性血清中精子相关抗原升高。通过三种不同的检测方法在部分输精管切除男性的血清中检测到了CIC。用聚乙二醇沉淀患者血清中的CIC，使其解离，并用酶联免疫吸附测定法鉴定各个CIC成分。大多数（但并非全部）CIC含有与抗精子免疫球蛋白（Ig）G发生反应的抗原，一些还含有补体成分C3和/或C1q。在一些IgG和精子抗原呈阳性的CIC中鉴定出了IgA，两名男性的CIC中含有IgM。通过蔗糖梯度离心法对CIC进行分析，结果显示其大小不均一。
