Alfa M, Lee A
J Clin Pathol. 1982 May;35(5):555-60. doi: 10.1136/jcp.35.5.555.
The need for separate swab transport methods for aerobes and anaerobes may result in inadequate transport of specimens for anaerobic bacteriology. Most microbiology laboratories in Australia rely on Stuart's transport medium to protect anaerobic bacteria. This paper presents a new, simple transport medium (Transport Deep) suitable for sue with aerobes and anaerobes. Comparative evaluations demonstrate that Transport Deep is as good as Stuart's medium for the maintenance of fastidious bacteria and is far superior for the protection of even extremely oxygen-sensitive anaerobes. This medium has been used successfully in a large Sydney hospital for more than a year. It is proposed that Transport Deep be used on a routine basis for all swab specimens.
需采用不同的拭子运送方法分别运送需氧菌和厌氧菌,这可能导致用于厌氧细菌学检测的标本运送不充分。澳大利亚的大多数微生物实验室都依赖于斯图尔特运送培养基来保护厌氧菌。本文介绍了一种新型、简单的运送培养基(运送深层培养基),适用于需氧菌和厌氧菌。对比评估表明,运送深层培养基在维持苛养菌方面与斯图尔特培养基效果相当,在保护甚至对氧气极度敏感的厌氧菌方面则远优于斯图尔特培养基。这种培养基已在悉尼一家大型医院成功使用了一年多。建议对所有拭子标本常规使用运送深层培养基。