Pedersen F S, Crowther R L, Hays E F, Nowinski R C, Haseltine W A
J Virol. 1982 Jan;41(1):18-29. doi: 10.1128/JVI.41.1.18-29.1982.
The retrovirus expression of eight independent lymphoid cell lines derived from spontaneous thymomas of AKR mice was investigated. The RNase T1 fingerprints of viral 70S RNA produced by these cell lines were compared with genome structures of the non-leukemogenic Akv virus and with two types of cloned leukemogenic viruses derived from one of the thymoma cell lines. Viral RNAs from three cell lines, SL3, 4, and 7, were indistinguishable from one another. The fingerprint patterns indicated that these cell lines produce equal amounts of two prototype, leukomogenic SL viruses that were previously isolated from the SL3 cell line. Viral RNA produced by the SL1 and SL2 cell lines contained similar components, but at a different ratio. Two other cell lines (SL5 and SL11) produced viral RNAs that resemble those of AKR mink cell focus-forming viruses. One additional line, SL9, produced viral RNA of a novel structure. The complex pattern of viral RNA expression observed for these lymphoid cell lines can be interpreted in terms of recombination among three types of endogenous viral sequences: the Akv virus, a xenotropic virus, and an SL (for spontaneous leukemia) virus.
对源自AKR小鼠自发性胸腺瘤的8个独立淋巴细胞系的逆转录病毒表达进行了研究。将这些细胞系产生的病毒70S RNA的核糖核酸酶T1指纹图谱与非致白血病的Akv病毒的基因组结构以及源自其中一个胸腺瘤细胞系的两种克隆致白血病病毒的基因组结构进行了比较。来自三个细胞系SL3、4和7的病毒RNA彼此无法区分。指纹图谱表明，这些细胞系产生等量的两种原型致白血病SL病毒，这两种病毒先前是从SL3细胞系中分离出来的。SL1和SL2细胞系产生的病毒RNA含有相似的成分，但比例不同。另外两个细胞系（SL5和SL11）产生的病毒RNA与AKR貂细胞集落形成病毒的RNA相似。另一个细胞系SL9产生了一种新型结构的病毒RNA。这些淋巴细胞系中观察到的病毒RNA表达的复杂模式可以用三种内源性病毒序列之间的重组来解释：Akv病毒、嗜异性病毒和SL（自发性白血病）病毒。
