Castro A E, Daley G G, Zimmer M A, Whitenack D L, Jensen J
Am J Vet Res. 1982 Jan;43(1):5-11.
Herpesviruses were isolated in bovine cell cultures from buffy coat cells obtained from an Indian gaur (Bos gaurus) and a greater kudu (Tragelaphus strepsiceros) with clinical signs of the head and eye form of malignant catarrhal fever (MCF). Both animals were from herds housed in a zoologic park in Oklahoma. Serial transmission of the head and eye form of MCF was accomplished by using whole blood from the gaur into a Hereford-Angus heifer, then whole blood from the heifer into a Holstein calf, and finally, whole blood from the calf into a white-tailed deer (Odocoileus virginianus). A herpesvirus was isolated in bovine cell cultures inoculated with buffy coat cells from the heifer, and 2 deer inoculated with this herpesvirus developed the head and eye form of MCF. A deer inoculated with whole blood from the greater kudu also developed clinical signs of MCF, and a herpesvirus was subsequently recovered from the deer. Clinical signs of MCF included a mucopurulent catarrh, pyrexia (38.8 to 42.1 C), anorexia, and corneal opacity, and death occurred between postinoculation days 15 and 21.
从患有头眼型恶性卡他热（MCF）临床症状的印度野牛（Bos gaurus）和大林羚（Tragelaphus strepsiceros）采集的血沉棕黄层细胞，在牛细胞培养物中分离出疱疹病毒。这两只动物均来自俄克拉何马州一个动物园的畜群。通过将野牛的全血注入一头海福特-安格斯小母牛，然后将小母牛的全血注入一头荷斯坦小牛，最后将小牛的全血注入一只白尾鹿（Odocoileus virginianus），实现了头眼型MCF的连续传播。从接种了小母牛血沉棕黄层细胞的牛细胞培养物中分离出一种疱疹病毒，接种这种疱疹病毒的2只鹿出现了头眼型MCF。接种大林羚全血的一只鹿也出现了MCF的临床症状，随后从该鹿体内分离出一种疱疹病毒。MCF的临床症状包括黏液脓性卡他、发热（38.8至42.1摄氏度）、厌食和角膜混浊，接种后第15至21天死亡。
