Al-Darraji A M, Cutlip R C, Lehmkuhl H D, Graham D L, Kluge J P, Frank G H
Am J Vet Res. 1982 Feb;43(2):236-40.
Four-week-old lambs were inoculated transtracheally with respiratory syncytial virus (RSV), Pasteurella haemolytica, or RSV and P haemolytica. When given in combination, RSV administration preceded P haemolytica by 3 or 5 days. Lambs inoculated with P haemolytica or RSV developed a mild respiratory tract disease accompanied by a transient pyrexia in a few lambs. By 24 hours after inoculation of bacteria, all lambs inoculated with RSV and P haemolytica were listless, reluctant to move, and exhibited hyperpnea and dyspnea. Most lambs had pyrexia and a few coughed and had serous nasal discharge. These clinical signs persisted for 3 to 4 days and were more pronounced in those inoculated with P haemolytica 5 days after RSV than in those inoculated with P haemolytica 3 days after RSV. Respiratory syncytial virus was isolated from 8 of 15 inoculated lambs and P haemolytica was isolated from 12 of 15 inoculated lambs. All lambs responded serologically to RSV, but none responded to P haemolytica.
给4周龄的羔羊经气管接种呼吸道合胞病毒（RSV）、溶血巴氏杆菌，或同时接种RSV和溶血巴氏杆菌。当联合接种时，RSV的接种比溶血巴氏杆菌提前3天或5天。接种溶血巴氏杆菌或RSV的羔羊出现轻度呼吸道疾病，少数羔羊伴有短暂发热。接种细菌后24小时，所有接种RSV和溶血巴氏杆菌的羔羊都无精打采、不愿活动，并表现出呼吸急促和呼吸困难。大多数羔羊发热，少数咳嗽并有浆液性鼻液。这些临床症状持续3至4天，在RSV接种5天后接种溶血巴氏杆菌的羔羊中比在RSV接种3天后接种溶血巴氏杆菌的羔羊中更为明显。从15只接种羔羊中的8只分离出呼吸道合胞病毒，从15只接种羔羊中的12只分离出溶血巴氏杆菌。所有羔羊对RSV均产生血清学反应，但对溶血巴氏杆菌均无反应。
