Clapham D E, DeFelice L J
J Membr Biol. 1982;67(1):63-71. doi: 10.1007/BF01868648.
The electrical impedance of seven-day ventricular embryonic chick heart cell membranes maintained in tissue culture was measured under voltage clamp using the two-microelectrode voltage-clamp technique. Small sinusoidal perturbations were added to the voltage-clamp potential and the amplitude and phase of the steady-state sinusoidal response in current was recorded as a function of mean clamp potential or perturbing frequency. The experimental results are compared with two models of excitability for heart: the MNT model (McAllister, Noble & Tsien, J. Physiol. (London) 251:1-59, (1975) and the BR model (Beeler & Reuter, J. Physiol. (London) 268:177-210, 1977). The small signal impedance of heart cell membranes, in theory and experiment, shows a resonance near 1 Hz and near the threshold potential. The effect of this resonance is to increase the effective length constant of the membrane for these conditions.
采用双微电极电压钳技术，在电压钳条件下测量了组织培养中维持7天的鸡胚心室心肌细胞膜的电阻抗。向电压钳电位施加小的正弦扰动，并记录电流中稳态正弦响应的幅度和相位，作为平均钳位电位或扰动频率的函数。将实验结果与心脏兴奋性的两种模型进行了比较：MNT模型（McAllister、Noble和Tsien，《生理学杂志》（伦敦）251:1 - 59，（1975年））和BR模型（Beeler和Reuter，《生理学杂志》（伦敦）268:177 - 210，1977年）。理论和实验表明，心肌细胞膜的小信号阻抗在1Hz附近和阈值电位附近出现共振。在这些条件下，这种共振的作用是增加膜的有效长度常数。
