Kahn S N, Thompson E J
Clin Chim Acta. 1978 Oct 16;89(2):253-65. doi: 10.1016/0009-8981(78)90323-6.
A new method is described which allows rapid identification of sub-microgram amounts of protein in polyacrylamide gels. The technique has been applied to samples of cerebrospinal fluid and serum and is suitable for localisation of both discrete protein bands and diffuse zones. Results from cerebrospinal fluid confirm that IgG is localised in the oligoclonal bands seen in multiple sclerosis. Given antibody excess, there is a linear relationship between the amount of protein applied to the gel and the density of the stained immune complexes on the gel surface.
本文描述了一种新方法，该方法可快速鉴定聚丙烯酰胺凝胶中亚微克量的蛋白质。该技术已应用于脑脊液和血清样本，适用于离散蛋白条带和弥散区的定位。脑脊液检测结果证实，免疫球蛋白G（IgG）定位于多发性硬化症患者脑脊液中的寡克隆带。在抗体过量的情况下，加样于凝胶上的蛋白量与凝胶表面染色免疫复合物的密度之间呈线性关系。
