Hansen C E, Dueland S, Drevon C A, Størmer F C
Appl Environ Microbiol. 1982 Jun;43(6):1267-71. doi: 10.1128/aem.43.6.1267-1271.1982.
Association of ochratoxin A with cultured rat hepatocytes occurs at 4 degrees C, and the saturation level in the medium is 0.3 mM ochratoxin A, with maximal binding after 60 min. At 37 degrees C the level of cell-associated ochratoxin A increased up to 6 h and remained at 2 nmol of toxin per mg of cell protein for 30 h. With increasing concentrations of ochratoxin A, increasing amounts of the toxin accumulated in the cells; saturation occurred at a concentration of 0.3 mM. Ochratoxin A was metabolized by hepatocytes at 37 degrees. (4R)-4-Hydroxyochratoxin A appeared in the medium at a maximal level (about 30 nmol/mg of cell protein) at an ochratoxin A concentration of 0.25 mM after 48 h of incubation. Small amounts of (4S)-4-hydroxyochratoxin A were detected only after incubation for 22 h or longer.
赭曲霉毒素A与培养的大鼠肝细胞的结合在4℃时发生，培养基中的饱和水平为0.3 mM赭曲霉毒素A，60分钟后达到最大结合。在37℃时，细胞相关的赭曲霉毒素A水平在6小时内升高，并在30小时内保持在每毫克细胞蛋白2 nmol毒素的水平。随着赭曲霉毒素A浓度的增加，细胞中积累的毒素量也增加；在0.3 mM的浓度下发生饱和。赭曲霉毒素A在37℃时被肝细胞代谢。孵育48小时后，在赭曲霉毒素A浓度为0.25 mM时，(4R)-4-羟基赭曲霉毒素A在培养基中的出现水平最高（约30 nmol/毫克细胞蛋白）。仅在孵育22小时或更长时间后才检测到少量的(4S)-4-羟基赭曲霉毒素A。
