Makler A
Fertil Steril. 1978 Sep;30(3):313-8. doi: 10.1016/s0015-0282(16)43518-1.
A new chamber for sperm count and motility estimation is described. This chamber, which is only 10 micron deep, enables free horizontal movement of spermatozoa in one focal plane and provides conditions for the examination of undiluted samples. Therefore, with the aid of this instrument it is possible to compare sperm motility in various samples from the same person or in different samples at different times. This can be done either by simple estimation or with any other method of motility evaluation chosen by the examiner. The sperm count can be made rapidly and directly from an undiluted, preheated sample by counting spermatozoa in the area of a grid located within the eyepiece; the count is expressed in millions per milliliter. Thirty-seven specimens were analyzed with this chamber. Statistical evaluation of the results revealed high precision, accuracy, and reliability of sperm counts when compared with the hemocytometric method. Better results were obtained when motility estimation was compared with the ordinary slide technique. Easy performance, rapid sperm counts, and improvement of motility estimation make this chamber a useful tool where sperm analysis is carried out.
本文描述了一种用于精子计数和活力评估的新型计数室。该计数室仅10微米深，能使精子在一个焦平面内自由水平移动，并为检测未稀释样本提供条件。因此，借助该仪器，可以比较同一人不同样本或不同时间不同样本中的精子活力。这可以通过简单估计或检查者选择的任何其他活力评估方法来完成。通过对位于目镜内网格区域的精子进行计数，可以快速直接地从未稀释、预热的样本中进行精子计数；计数结果以每毫升百万计。使用该计数室对37个样本进行了分析。结果的统计评估显示，与血细胞计数法相比，精子计数具有很高的精密度、准确性和可靠性。与普通载玻片技术相比，活力评估获得了更好的结果。操作简便、精子计数快速以及活力评估的改进，使该计数室成为进行精子分析的有用工具。
