Rybak J, Tharapel A, Robinett S, Garcia M, Mankinen C, Freeman M
Hum Genet. 1982;60(4):328-33. doi: 10.1007/BF00569213.
A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase.
描述了一种分析前期和早中期染色体的方法。该方法包括在添加10%自体血浆而非胎牛血清的RPMI-1640培养基中培养淋巴细胞。收获前1小时，用放线菌素D和秋水仙酰胺处理活细胞，并在培养65小时时提前收获，使用大贯（1968年）配制的低渗溶液。该方法已在数百份临床样本上进行了常规测试。平均而言，30%的分裂细胞处于前中期。
