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通过免疫电泳检测“中性”7F型和14型肺炎球菌荚膜多糖

Detection of "neutral" type 7F and type 14 pneumococcal capsular polysaccharides by immunoelectrophoresis.

作者信息

Szu S C, Oravec L S

出版信息

J Clin Microbiol. 1982 Jun;15(6):1172-5. doi: 10.1128/jcm.15.6.1172-1175.1982.

Abstract

Type 7F and type 14 pneumococcal capsular polysaccharides, neutral at pH 8.6, were studied by immunoelectrophoresis at pH 5. Three techniques were used: rocket, countercurrent, and reversed immunoelectrophoresis. Our results show that these two capsular polysaccharides types can be detected at pH 5 with high sensitivity.

摘要

对在pH 8.6呈中性的7F型和14型肺炎球菌荚膜多糖在pH 5条件下进行了免疫电泳研究。使用了三种技术:火箭免疫电泳、对流免疫电泳和反向免疫电泳。我们的结果表明,这两种荚膜多糖类型在pH 5时能够被高灵敏度检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c20/272277/fb650a167ff3/jcm00155-0227-a.jpg

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本文引用的文献

6
Quantitation of diphtheria and tetanus antibodies by reversed rocket immunoelectrophoresis.
J Immunol Methods. 1975 Feb;6(4):385-98. doi: 10.1016/0022-1759(75)90009-5.

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