用多价对流免疫电泳（PIE）进行肺炎球菌分型。

Pneumococcal typing by polyvalent counterimmunoelectrophoresis (PIE).

作者信息

Holliday M G

出版信息

J Immunol Methods. 1981;46(2):243-9. doi: 10.1016/0022-1759(81)90140-x.

Abstract

A one-step polyvalent counterimmunoelectrophoresis (PIE) method for the typing of pneumococci is describe. Only one antiserum is used (omniserum, containing antibodies to greater than 80 pneumococcal types) and it is not necessary to stock large numbers of monospecific typing sera. By observing reactions of identity between the precipitin lines produced by the pneumococcus under test, and the specific capsular polysaccharide precipitin lines in a reference pattern produced by the polyvalent pneumococcal vaccine 'Pneumovax', a cumulative percentage of 64.6% of pneumococcal types isolated could be typed. The method is simple, reproducible, inexpensive and provides a permanent stained record.

摘要

描述了一种用于肺炎球菌分型的一步多价对流免疫电泳（PIE）方法。仅使用一种抗血清（全血清，含针对80多种肺炎球菌型别的抗体），无需储备大量单价分型血清。通过观察待测肺炎球菌产生的沉淀线与多价肺炎球菌疫苗“肺炎球菌多糖疫苗”产生的参考模式中的特异性荚膜多糖沉淀线之间的同一性反应，可对分离出的64.6%的肺炎球菌型别进行分型。该方法简单、可重复、成本低，并提供永久性染色记录。