Borges S, Lewis P D
Teratology. 1982 Jun;25(3):283-9. doi: 10.1002/tera.1420250304.
Pregnant Wistar rats were maintained on a diet of food pellets and a 10% (v/v) solution of ethanol in water during pregnancy and up to 21 days postpartum. Control groups were given food pellets and water ad libitum. The consumption of food and liquid was monitored throughout this period, so that daily food- and ethanol-derived caloric intake, daily liquid intake, and dose of alcohol ingested could be determined. The body weights and whole brain weights of ethanol-exposed offspring did not differ significantly from controls at any time from birth up to 21 days postpartum. However, cerebellar weights were significantly reduced in 21-day-old ethanol-exposed offspring. Areal analysis of the cerebellar vermis showed a 9% reduction in 21-day-old ethanol-exposed offspring compared with controls. Although there were no differences in total vermal area between control and treated rats at 12 days, ethanol-exposed offspring had a significantly more extensive external granular layer. In midgestation, pregnant females registered peak alcohol levels of 118 mg/100 ml blood 1 hr prior to the end of the dark cycle, while during lactation peak levels of 135 mg/100 ml blood were obtained. Neonate alcohol levels peaked slightly later and reached much lower values (44 mg/100 ml blood) during the lactation period. Ingested alcohol intake and blood alcohol levels fall within the range associated in man with the fetal alcohol syndrome, and it is suggested that the experimental regime described is a useful method for investigating the effects of alcohol on the developing nervous system.
妊娠期间及产后21天，将妊娠的Wistar大鼠饲养在含有食物颗粒和10%（体积/体积）乙醇水溶液的饮食中。对照组随意给予食物颗粒和水。在此期间监测食物和液体的摄入量，以便确定每日食物和乙醇的热量摄入、每日液体摄入量以及摄入的酒精剂量。从出生到产后21天的任何时候，暴露于乙醇的后代的体重和全脑重量与对照组相比均无显著差异。然而，21日龄暴露于乙醇的后代的小脑重量显著降低。对小脑蚓部的面积分析显示，与对照组相比，21日龄暴露于乙醇的后代减少了9%。虽然在12天时，对照大鼠和处理大鼠的总蚓部面积没有差异，但暴露于乙醇的后代的外颗粒层明显更广泛。在妊娠中期，妊娠雌性大鼠在黑暗周期结束前1小时的血液酒精峰值水平为118 mg/100 ml，而在哺乳期，血液酒精峰值水平为135 mg/100 ml。新生儿酒精水平在哺乳期稍晚达到峰值，且值低得多（44 mg/100 ml血液）。摄入的酒精量和血液酒精水平处于与人类胎儿酒精综合征相关的范围内，建议所描述的实验方案是研究酒精对发育中神经系统影响的有用方法。
