Gross G N, Rehm S R, Toews G B, Hart D A, Pierce A K
Infect Immun. 1978 Jul;21(1):7-9. doi: 10.1128/iai.21.1.7-9.1978.
We have studied the clearance from murine lungs of two strains of Staphylococcus aureus, one possessing high and the other possessing low levels of protein A.S. aureus FDA 209 and S. aureus Wood 46 were assayed for their ability to bind mouse immunoglobulin G, using an indirect radioimmunoassay. S. aureus FDA 209 binding of mouse immunoglobulin was significantly greater than that of S. aureus Wood 46 (118,909 versus 37,845 cpm). Clearance of these two strains from the lung after a 30-min aerosol inoculation period was not significantly different. The percentage of bacteria remaining in the lung was 49.2 and 55.0% at 2h, 31.8 and 33.2% at 3 h, and 25.4 and 17.2% at 4 h for protein A-rich and protein A-poor strains, respectively (P greater than 0.20 at each time). These data suggest that the previously demonstrated in vitro antiphagocytic effect of protein A may not be relevant to pulmonary clearance mechanisms.
我们研究了两种金黄色葡萄球菌菌株从鼠肺中的清除情况，一种菌株的蛋白A水平高，另一种菌株的蛋白A水平低。使用间接放射免疫分析法检测了金黄色葡萄球菌FDA 209和金黄色葡萄球菌Wood 46结合小鼠免疫球蛋白G的能力。金黄色葡萄球菌FDA 209对小鼠免疫球蛋白的结合能力显著高于金黄色葡萄球菌Wood 46（分别为118,909和37,845 cpm）。在30分钟的气溶胶接种期后，这两种菌株从肺中的清除情况没有显著差异。富含蛋白A和蛋白A缺乏的菌株在2小时时肺中残留细菌的百分比分别为49.2%和55.0%，在3小时时分别为31.8%和33.2%，在4小时时分别为25.4%和17.2%（每次P均大于0.20）。这些数据表明，先前证明的蛋白A的体外抗吞噬作用可能与肺部清除机制无关。
