Borenstein D G, Gibbs C A, Jacobs R P
Arthritis Rheum. 1982 Aug;25(8):947-53. doi: 10.1002/art.1780250806.
Nonvolatile short-chain fatty acids from 80 synovial fluids were quantified by gas-liquid chromatography. Succinic acid was detectable in all 23 septic synovial fluids infected with either gram-positive or gram-negative organisms and in only 5 of 57 nonseptic synovial fluids. Lactic acid was present in all of the effusions but was correlated with septic arthritis only when present in concentrations greater than 250 mg%. Neither short-chain fatty acid was more sensitive than high white blood cell counts (greater than 50,000 mm3) or depressed glucose concentration (less than 40 mg/dl) in diagnosing septic arthritis before antibiotic therapy; however, the detection of succinic acid was helpful in identifying patients with septic arthritis who had been given antibiotic treatment before arthrocentesis. Thus, gas-liquid chromatography, a rapid and sensitive method for the detection of short-chain fatty acids, may complement the currently available methods used to diagnose septic arthritis.
采用气液色谱法对80份滑液中的非挥发性短链脂肪酸进行定量分析。在所有23份感染革兰氏阳性或革兰氏阴性菌的化脓性滑液中均能检测到琥珀酸，而在57份非化脓性滑液中只有5份能检测到。所有积液中均存在乳酸，但只有当乳酸浓度大于250mg%时才与化脓性关节炎相关。在抗生素治疗前诊断化脓性关节炎时，这两种短链脂肪酸均不比高白细胞计数（大于50,000/mm³）或血糖浓度降低（小于40mg/dl）更敏感；然而，琥珀酸的检测有助于识别在关节穿刺术前已接受抗生素治疗的化脓性关节炎患者。因此，气液色谱法作为一种检测短链脂肪酸的快速灵敏方法，可能会补充目前用于诊断化脓性关节炎的现有方法。
