Brook I, Reza M J, Bricknell K S, Bluestone R, Finegold S M
Ann Rheum Dis. 1980 Apr;39(2):168-72. doi: 10.1136/ard.39.2.168.
Gas liquid chromatography was performed on synovial fluid of 94 patients with exudative arthritis. A relatively constant pattern of peaks was obtained in all synovial fluid samples. Lactic acid was increased in synovial fluid of patients with septic arthritis excluding gonococcal arthritis. In addition 2 constant peaks with retention time of 546 and 848 seconds in the chromatogram usually showed a significant increase in the synovial fluids of patients with septic arthritis (including gonococcal arthritis) but not in those from patients with sterile inflammatory or degenerative arthritis. In 1 patient with pseudomonas arthritis an increase in the peaks was noted with clinical and bacteriological relapse and subsequently there was a gradual return to normal levels during clinical improvement. Increase in synovial fluid lactic acid is useful in the diagnosis of septic arthritis. Identification of the compounds represented by the 2 peaks, which presumptively correspond to n-valeric and n-hexanoic acid, may provide further information on diagnosis, prognosis and pathogenesis of arthritis and may help in the differentiation between gonococcal and nongonococcal arthritis.
对94例渗出性关节炎患者的滑液进行了气相液相色谱分析。在所有滑液样本中均获得了相对恒定的峰型。除淋菌性关节炎外，化脓性关节炎患者滑液中的乳酸含量升高。此外，色谱图中保留时间为546秒和848秒的2个恒定峰，在化脓性关节炎（包括淋菌性关节炎）患者的滑液中通常显著升高，但在无菌性炎症或退行性关节炎患者的滑液中则不然。1例假单胞菌性关节炎患者在临床和细菌学复发时峰升高，随后在临床改善过程中逐渐恢复至正常水平。滑液乳酸升高对化脓性关节炎的诊断有帮助。鉴定这2个峰所代表的化合物（推测分别对应正戊酸和正己酸），可能会为关节炎的诊断、预后和发病机制提供更多信息，并有助于区分淋菌性和非淋菌性关节炎。
