Rowe D A, Fallon J F
J Embryol Exp Morphol. 1982 Jun;69:1-6.
Impermeable barriers were inserted into the stage-20 to -21 leg bud to test whether or not such an interruption of diffusion of the proposed morphogen from the polarizing zone would result in failure of leg elements to develop anterior to the barrier. Tantalum foil was placed at somite levels 30/31 or mid-31 through the dorsoventral extent of the leg bud separating anterior from posterior mesoderm and ectodermal ridge. In the resulting legs, structures developed anterior to the level of the barrier. For example, legs with foil at the 30-/31-somite level developed digits 1 and 2. We conclude that either the barrier is not an effective block of diffusion of polarizing zone morphogen or that the influence of the polarizing zone is not required for determination of leg structures at these stages.
将不可渗透的屏障插入到20至21期的腿芽中，以测试这种对来自极化区的假定形态发生素扩散的阻断是否会导致腿元件在屏障前方发育失败。钽箔放置在第30/31体节水平或第31体节中部，穿过腿芽的背腹范围，将前后中胚层和外胚层嵴分开。在形成的腿中，结构在屏障水平前方发育。例如，在第30/31体节水平放置箔片的腿发育出了第1和第2趾。我们得出结论，要么屏障不是极化区形态发生素扩散的有效阻断物，要么在这些阶段确定腿部结构不需要极化区的影响。
