Abdelnoor A M, Harvie N R, Johnson A G
Infect Immun. 1982 Oct;38(1):157-61. doi: 10.1128/iai.38.1.157-161.1982.
Normal human serum and a fraction rich in lipoprotein, Cohn fraction IV1, have been shown in previous studies to detoxify native endotoxin by decreasing lethality for mice, fever in rabbits, and by the alteration of the characteristic endotoxin-anti-endotoxin precipitin pattern in gels. These studies are extended herein and document the ability of normal human serum and fraction IV1 to neutralize the induction of hypotension in rabbits by viable gram-negative bacilli. Further fractionation of serum, using an ultracentrifugal flotation method for producing lipoprotein-free human serum and purified high-density lipoproteins, revealed the lipoprotein-free fraction to be capable of inhibiting endotoxin hypotensive activity and to alter diffusion of endotoxin in gels. On the other hand, the purified high-density lipoproteins failed to negate either activity.
在先前的研究中已表明，正常人血清和富含脂蛋白的组分（科恩组分IV1）可通过降低对小鼠的致死率、兔子的发热反应以及改变凝胶中内毒素-抗内毒素沉淀素特征模式来解毒天然内毒素。本文扩展了这些研究，并证明了正常人血清和组分IV1能够中和活的革兰氏阴性杆菌对兔子诱导的低血压。使用超速离心浮选法对血清进行进一步分级分离，以制备无脂蛋白的人血清和纯化的高密度脂蛋白，结果显示无脂蛋白组分能够抑制内毒素的降压活性并改变内毒素在凝胶中的扩散。另一方面，纯化的高密度脂蛋白未能消除任何一种活性。
