Wilks M, Thin R N, Tabaqchali S
J Med Microbiol. 1982 Feb;15(1):141-7. doi: 10.1099/00222615-15-1-141.
Three recently described methods for quantitative sampling of the bacterial flora of the vagina were evaluated and none proved satisfactory. In a third of the samples, paired swabs showed large differences between the two weights of vaginal secretion collected by this method, and the recovery rate of bacteria deliberately added to test swabs was unsatisfactorily low. A calibrated loop gave a wide variation in the amount of secretion collected, due to variations in density and viscosity of the secretion. When secretion was collected with a calibrated pipette, it was often difficult to expel the collected volume from the pipette for testing. The simple weight-based method was devised in which a loop was used to collect an undefined volume of secretion for weighting in a tube of transport medium before homogenisation and quantitative bacteriological testing. Initial assessment indicates this to be a satisfactory method for quantitative studies of the vaginal bacterial flora.
对最近描述的三种阴道细菌菌群定量采样方法进行了评估，结果均不尽人意。在三分之一的样本中，配对拭子显示通过该方法收集的两份阴道分泌物重量差异很大，故意添加到测试拭子中的细菌回收率低得令人不满意。校准环收集的分泌量差异很大，这是由于分泌物的密度和粘度不同所致。当用校准移液器收集分泌物时，往往很难将收集的体积从移液器中挤出进行测试。设计了一种基于简单重量的方法，即使用环收集不确定体积的分泌物，在均质化和定量细菌学检测之前在一管运输培养基中称重。初步评估表明，这是一种用于阴道细菌菌群定量研究的令人满意的方法。
