Masfari A N, Duerden B I, Kinghorn G R
Genitourin Med. 1986 Aug;62(4):256-63. doi: 10.1136/sti.62.4.256.
A quantitative method of culture, based on a weighed sample and with results expressed as colony forming units (cfu)/g was assessed and used to investigate the vaginal flora of normal women and that of women with vaginal disease. Samples were collected by means of disposable plastic loops into modified proteose peptone water transport medium in preweighed bottles. Counts expressed as cfu/g of secretion were consistent, whereas counts expressed as cfu/ml were inconsistent. Results obtained with specimens manipulated on the open bench were the same as those from duplicate samples processed in an anaerobic chamber. The normal vaginal flora was predominantly aerobic--lactobacilli, coryneforms, and coagulase negative staphylococci--with counts of greater than or equal to 10(8) cfu/g for lactobacilli. These were also present in patients with candidosis, but the flora in patients with trichomoniasis, bacterial vaginosis, gonorrhoea, or chlamydial infection was predominantly anaerobic. The commonest anaerobes were Bacteroides spp, particularly B bivius; they were found in 55% of controls but at counts of 10(2) cfu/g lower than in the patients, most of whom had high counts of anaerobes (greater than 10(8) cfu/g). The isolation rate of Gardnerella vaginalis was not appreciably greater from patients with bacterial vaginosis, and the quantitative cultures on controls and patients who were G vaginalis positive were the same (approximately equal to 10(7) cfu/g). Quantitative studies show greater differences than qualitative cultures between normal controls and patients with vaginal infections, indicating that some symptoms and signs of such infections may be related to quantitative polymicrobial changes.
一种基于称重样本且结果以菌落形成单位（cfu）/克表示的定量培养方法被评估并用于研究正常女性和患有阴道疾病女性的阴道菌群。通过一次性塑料环将样本收集到预先称重瓶中的改良蛋白胨水转运培养基中。以cfu/克分泌物表示的计数是一致的，而以cfu/毫升表示的计数则不一致。在开放实验台上处理的标本所获得的结果与在厌氧箱中处理的重复样本的结果相同。正常阴道菌群主要是需氧菌——乳酸杆菌、棒状杆菌和凝固酶阴性葡萄球菌——乳酸杆菌计数大于或等于10⁸ cfu/克。念珠菌病患者中也存在这些菌，但滴虫病、细菌性阴道病、淋病或衣原体感染患者的菌群主要是厌氧菌。最常见的厌氧菌是拟杆菌属，尤其是二路拟杆菌；在55%的对照中发现了它们，但计数比患者低10² cfu/克，大多数患者的厌氧菌计数较高（大于10⁸ cfu/克）。细菌性阴道病患者中阴道加德纳菌的分离率并没有明显更高，对照和阴道加德纳菌阳性患者的定量培养结果相同（约10⁷ cfu/克）。定量研究表明，正常对照与阴道感染患者之间的差异在定量培养中比定性培养中更大，这表明此类感染的一些症状和体征可能与定量的多微生物变化有关。
