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[静态和动态培养后大鼠海马的形态计量学研究]

[Morphometric studies of the rat hippocampus after static and dynamic cultivation].

作者信息

Lindner G, Grosse G

出版信息

Z Mikrosk Anat Forsch. 1982;96(3):485-96.

PMID:7148093
Abstract

Explants of the hippocampus (rat) were cultivated either in maximow chambers without movement (static) or in movement (dynamic) by rotation in roller tubes. The growth of nerve fibres, the density of cells (matrix, neurons) and the area of cell nuclei was observed. The determination of the index of nerve fibre growth, the procedure for counting cells in a tissue with different densities and the value of the two methodological approaches of cultivation is discussed. Finally it is stated that best results in morphology of explants can only be obtained by cultivation without movement.

摘要

将大鼠海马体的外植体分别培养在无运动的马克西莫培养箱(静态)中,或通过在滚瓶中旋转进行运动培养(动态)。观察神经纤维的生长、细胞(基质、神经元)密度和细胞核面积。讨论了神经纤维生长指数的测定、在不同密度组织中计数细胞的方法以及两种培养方法的价值。最后指出,只有通过无运动培养才能在海马体外植体形态学上获得最佳结果。

相似文献

1
[Morphometric studies of the rat hippocampus after static and dynamic cultivation].[静态和动态培养后大鼠海马的形态计量学研究]
Z Mikrosk Anat Forsch. 1982;96(3):485-96.
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[Effect of the culture technic on the development of the hippocampus (rat) in vitro].[培养技术对体外海马体(大鼠)发育的影响]
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[The effect of substance P on the regeneration of nerve fibres in vitro (author's transl)].P物质对体外神经纤维再生的作用(作者译)
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[Effect of substance P on nerve fiber regeneration in tissue culture].[P物质对组织培养中神经纤维再生的影响]
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