Lindner G, Grosse G
Z Mikrosk Anat Forsch. 1982;96(3):485-96.
Explants of the hippocampus (rat) were cultivated either in maximow chambers without movement (static) or in movement (dynamic) by rotation in roller tubes. The growth of nerve fibres, the density of cells (matrix, neurons) and the area of cell nuclei was observed. The determination of the index of nerve fibre growth, the procedure for counting cells in a tissue with different densities and the value of the two methodological approaches of cultivation is discussed. Finally it is stated that best results in morphology of explants can only be obtained by cultivation without movement.
将大鼠海马体的外植体分别培养在无运动的马克西莫培养箱(静态)中,或通过在滚瓶中旋转进行运动培养(动态)。观察神经纤维的生长、细胞(基质、神经元)密度和细胞核面积。讨论了神经纤维生长指数的测定、在不同密度组织中计数细胞的方法以及两种培养方法的价值。最后指出,只有通过无运动培养才能在海马体外植体形态学上获得最佳结果。