Legakis N J, Xanthopoulou K, Ioannidou H, Papavassiliou J
Ann Microbiol (Paris). 1982 Sep-Oct;133(2):281-90.
Acidic metabolic products determined directly in clinical specimens of 51 anaerobic and 12 aerobic infections by quantitative gas-liquid chromatography (GLC) were correlated with the bacteriologic findings. The presence of appreciable amounts of succinic acid, more than 1 microM per ml, propionic and isovaleric acid, at concentrations not exceeding 3 microM per ml, was strong evidence for Bacteroides fragilis infections. When isobutyric acid was also detected, a B. melaninogenicus infection could be presumed. However, the presence of other anaerobes or aerobes could not be excluded. The presence of Clostridium spp. could also be presumed by the detection of butyric acid at a concentration greater than 4 microM per ml and of acetic, propionic and isobutyric acid at relatively high concentrations. In specimens from aerobic infections, no other acid except acetic and lactic acid could be detected. Our data also show that quantitative GLC is not valid in Peptococcus and Peptostreptococcus spp. infections except in some cases where Peptococcus alone or together with aerobic organisms are found.
采用定量气液色谱法（GLC）直接测定51例厌氧菌感染和12例需氧菌感染临床标本中的酸性代谢产物，并将其与细菌学检查结果进行关联。每毫升标本中琥珀酸含量超过1微摩尔、丙酸和异戊酸浓度不超过每毫升3微摩尔，这些显著量的存在是脆弱拟杆菌感染的有力证据。若同时检测到异丁酸，则可推测为产黑色素拟杆菌感染。然而，不能排除存在其他厌氧菌或需氧菌。每毫升标本中丁酸浓度大于4微摩尔，同时乙酸、丙酸和异丁酸浓度相对较高时，也可推测存在梭菌属。在需氧菌感染的标本中，除乙酸和乳酸外，未检测到其他酸。我们的数据还表明，定量GLC在消化球菌属和消化链球菌属感染中无效，除非在某些仅发现消化球菌或消化球菌与需氧菌同时存在的情况下。
