细胞合成抑制剂和紫外线照射对桦褐孔菌RNA诱导干扰素的影响。

The influence of inhibitors of cellular synthesis and UV irradiation on interferon induction by RNA from Piptoporus betulinus.

作者信息

Kandefer-Szerszeń M, Karpińska T, Kawecki Z

出版信息

Arch Immunol Ther Exp (Warsz). 1982;30(1-2):25-31.

Abstract

The effect of cycloheximide, chloramphenicol, ethidium bromide (EB), aurin tricarboxylic acid (ATA), and actinomycin D on the production of interferon (IFN) in human embryo fibroblasts (HAT) and L929 cells, stimulated with RNA from Piptoporus betulinus (Pb-RNA) was studied. Treatment of HAT cells with cycloheximide superinduced the production of IFN. Subsequent treatment with cycloheximide and actinomycin D chloramphenicol and actinomycin D or ATA and actinomycin D early after induction also enhanced the production of interferon, but the highest titers of interferon were observed when HAT cells were superinduced with cycloheximide and actinomycin D 11 hours after induction. In L929 cells the superinduction was efficient shortly after induction, but the IFN titers obtained were still very low. The effect of UV irradiation of HAT cells was also examined, but no enhancement of IFN production after induction with Pb-RNA was observed. Pb-RNA produced IFN in the spleen and liver of Swiss mice but no enhancement of IFN production was observed after treatment of mice with cycloheximide, ATA and EB.

摘要

研究了环己酰亚胺、氯霉素、溴化乙锭（EB）、金精三羧酸（ATA）和放线菌素D对经桦褐孔菌RNA（Pb - RNA）刺激的人胚成纤维细胞（HAT）和L929细胞中干扰素（IFN）产生的影响。用环己酰亚胺处理HAT细胞可超诱导IFN的产生。诱导后早期用环己酰亚胺和放线菌素D、氯霉素和放线菌素D或ATA和放线菌素D进行后续处理也可增强干扰素的产生，但当HAT细胞在诱导后11小时用环己酰亚胺和放线菌素D进行超诱导时，观察到干扰素的滴度最高。在L929细胞中，诱导后不久超诱导是有效的，但获得的IFN滴度仍然非常低。还检测了紫外线照射HAT细胞的效果，但在用Pb - RNA诱导后未观察到IFN产生的增强。Pb - RNA在瑞士小鼠的脾脏和肝脏中产生IFN，但在用环己酰亚胺、ATA和EB处理小鼠后未观察到IFN产生的增强。