Movshev B E, Averchenko V I
Biull Eksp Biol Med. 1982 Dec;94(12):33-5.
Blood serum proteins from normal and burnt rats (15-20% of the body surface) were fractionated with ammonium sulfate and gel filtration on Sephadex G-200. Analysis of the composition was performed by disc electrophoresis in 7.5% polyacrylamide gel. Blood serum of burnt rats is characterized by alterations in the relative content of protein fractions as compared with normal rats' serum. Additional components appearing after burns are absent from normal serum and differ from burn skin toxin. According to the physiochemical analysis data, no component corresponding to burn toxin could be found either in the whole serum or in the fractionation products.
对正常大鼠和体表烧伤面积为15 - 20%的烧伤大鼠的血清蛋白,用硫酸铵分级分离并在葡聚糖G - 200上进行凝胶过滤。通过在7.5%聚丙烯酰胺凝胶中进行圆盘电泳来分析其组成。与正常大鼠血清相比,烧伤大鼠血清的特征在于蛋白质组分的相对含量发生了变化。烧伤后出现的额外成分在正常血清中不存在,且与烧伤皮肤毒素不同。根据理化分析数据,在全血清或分级分离产物中均未发现与烧伤毒素相对应的成分。