Vesterinen E H, Nedrud J G, Collier A M, Walton L A, Pagano J S
Cancer Res. 1980 Mar;40(3):512-8.
Human ectocervical explant cultures were grown in medium with D-valine substituted for L-valine. Pure epithelial cell monolayers were obtained with both dialyzed and undialyzed fetal calf serum. Epithelial cell explant colonies grown in D-valine medium supplemented with undialyzed serum could routinely be subcultured if plated at a density of 1.5 x 10(4) cells/sq cm or higher. Such cultures could be passaged at least three times and could yield up to 21 population doublings per culture lifetime. Contaminating fibroblastic colonies were never detected in these cultures, which were free of surface-associated fibronectin as revealed by immunofluorescent tests. Both primary and passaged epithelial colonies retained many characteristic morphological features of ectocervical epithelium when examined by light and electron microscopy. Such cultures may be of use in investigating the action of viral and chemical carcinogenic agents upon epithelial cells in vitro.
人宫颈外植体培养物在以D - 缬氨酸替代L - 缬氨酸的培养基中生长。使用透析和未透析的胎牛血清均可获得纯上皮细胞单层。如果以1.5×10⁴个细胞/平方厘米或更高的密度接种，在补充未透析血清的D - 缬氨酸培养基中生长的上皮细胞外植体集落通常可以传代培养。这样的培养物可以传代至少三次，并且每个培养周期可产生多达21次群体倍增。在这些培养物中从未检测到污染的成纤维细胞集落，免疫荧光试验显示这些培养物没有表面相关的纤连蛋白。通过光学显微镜和电子显微镜检查时，原代和传代的上皮集落都保留了宫颈外上皮的许多特征性形态特征。这样的培养物可能有助于体外研究病毒和化学致癌剂对上皮细胞的作用。
