Bevan M, Barnes W M, Chilton M D
Nucleic Acids Res. 1983 Jan 25;11(2):369-85. doi: 10.1093/nar/11.2.369.
We present the DNA sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pTi T37 DNA from the virulent Agrobacterium tumefaciens strain T37. This region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. The strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.
我们展示了来自强致病力根癌土壤杆菌菌株T37的pTi T37 DNA右边界区域约2400个碱基对的DNA序列和植物肿瘤转录模式。该区域包括包含胭脂碱合成酶基因的整个转录单元，以及其他转录单元的部分区域。用于确定序列的策略还在目标DNA区域产生了两个相反的、确定的不对称缺失系列，其中一些可能在未来利用该序列时发挥作用，已知该序列在多种宿主植物组织中表达。
