Watanabe T, Yokoyama S, Hayashi K, Kasai H, Nishimura S, Miyazawa T
FEBS Lett. 1982 Dec 27;150(2):434-8. doi: 10.1016/0014-5793(82)80784-9.
Non-covalent DNA-binding has been studied of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (Me-IQ) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (Me-IQx), strong mutagens found in broiled foods. These mutagens are intercalated into DNA, as found by ultraviolet absorption and gel electrophoresis. The binding of IQ is stronger with GC pairs than AT pairs in DNA. The binding constants with calf thymus DNA are 1.6 X 10(6) (Me-IQ), 0.9 X 10(6) (IQ) and 0.7 X 10(6) M-1 (Me-IQx) at pH 6.0. This order of DNA affinity agrees with the order of mutagenicity towards Salmonella typhimurium TA98.
对2-氨基-3-甲基咪唑并[4,5-f]喹啉（IQ）、2-氨基-3,4-二甲基咪唑并[4,5-f]喹啉（Me-IQ）和2-氨基-3,8-二甲基咪唑并[4,5-f]喹喔啉（Me-IQx）的非共价DNA结合进行了研究，这些物质是在烤制食品中发现的强诱变剂。通过紫外吸收和凝胶电泳发现，这些诱变剂可插入DNA中。在DNA中，IQ与GC碱基对的结合比与AT碱基对的结合更强。在pH 6.0时，与小牛胸腺DNA的结合常数分别为1.6×10⁶（Me-IQ）、0.9×10⁶（IQ）和0.7×10⁶ M⁻¹（Me-IQx）。这种DNA亲和力的顺序与对鼠伤寒沙门氏菌TA98的诱变性顺序一致。
