Holme J A, Alexander J, Dybing E
Department of Toxicology, National Institute of Public Health, Oslo, Norway.
Cell Biol Toxicol. 1987 Mar;3(1):51-61. doi: 10.1007/BF00117825.
The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 times less than the activity with a subcellular fraction from rat liver. The mutagenic activity of both compounds was considerably increased using intestinal, renal and hepatic preparations isolated from PCB (Aroclor 1254)-pretreated rats, compared to preparations from control animals. In addition, both compounds displayed a moderate direct-acting mutagenic activity at concentrations above 10(-5) M. Isolated cells from small intestine, kidney and liver incubated in nucleopore chambers were able to convert both compounds into products which mutated bacteria outside the chambers. The concentrations of chemicals required to yield responses of a similar magnitude were approximately 3 orders of magnitude higher in the intestinal and renal systems compared to the hepatic system. The formation of metabolites mutagenic for Salmonella typhimurium by hepatic subcellular and cellular systems was shown to be superior to the respective intestinal and renal systems.
以大鼠肠道和肾脏亚细胞部分作为活化系统，在鼠伤寒沙门氏菌TA98中，热解产物2-氨基-3-甲基咪唑[4,5-f]喹啉和2-氨基-3,4-二甲基咪唑[4,5-f]喹啉的诱变活性分别约为每纳摩尔1个和5个回复突变体。这比用大鼠肝脏亚细胞部分测得的活性低1000倍。与对照动物的制剂相比，使用从多氯联苯（Aroclor 1254）预处理大鼠分离得到的肠道、肾脏和肝脏制剂时，这两种化合物的诱变活性都显著增加。此外，两种化合物在浓度高于10⁻⁵ M时都表现出中等程度的直接诱变活性。在核孔小室中培养的小肠、肾脏和肝脏分离细胞能够将这两种化合物转化为使小室外细菌发生突变的产物。与肝脏系统相比，在肠道和肾脏系统中产生类似程度反应所需的化学物质浓度大约高3个数量级。肝脏亚细胞和细胞系统对鼠伤寒沙门氏菌产生诱变代谢物的能力优于相应的肠道和肾脏系统。
