Allen G
Biochem J. 1982 Dec 1;207(3):397-408. doi: 10.1042/bj2070397.
The chromatographic properties of human interferon-alpha from Namalwa lymphoblastoid cells on Sephadex G-75 are described. The interferons are separated into two groups of four, with apparent molecular weights 19050 and 22000. Some of the latter form dimers at high concentrations. Fractions containing interferon were studied by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. Seven of the components had apparent molecular weights in this system, after reduction, of between 18400 and 20900: one component is probably glycosylated and has an apparent molecular weight of 27500. Amino acid sequences of peptides derived from interferon mixtures were determined and are related to published sequences deduced from the nucleotide sequences of cloned complementary DNA coding for interferon-alpha. The results show that the major interferon-alpha species isolated from Namalwa cells do not undergo C-terminal processing. Amino acid analyses of peptides are presented in Supplementary Publication SUP 50117 (28 pages), which has been deposited with the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1981) 193, 5.
本文描述了从Namalwa淋巴母细胞中提取的人α干扰素在Sephadex G - 75上的色谱特性。干扰素被分为两组，每组四个，其表观分子量分别为19050和22000。后者中的一些在高浓度时会形成二聚体。含有干扰素的组分在十二烷基硫酸钠存在下通过聚丙烯酰胺凝胶电泳进行研究。在该系统中，还原后七个组分的表观分子量在18400至20900之间：其中一个组分可能是糖基化的，其表观分子量为27500。测定了从干扰素混合物衍生的肽的氨基酸序列，并将其与从编码α干扰素的克隆互补DNA的核苷酸序列推导的已发表序列相关联。结果表明，从Namalwa细胞中分离出的主要α干扰素种类不进行C末端加工。肽的氨基酸分析见补充出版物SUP 50117（共28页），该出版物已存放在英国西约克郡韦瑟比波士顿温泉市的大英图书馆出借部，其馆藏编号为LS23 7BQ，可按《生物化学杂志》（1981年）第193卷第5期所示条件获取副本。
