Rzedzicki J, Gliński Z, Wernicki A
Pol Arch Weter. 1982;23(2):101-9.
The level of immunoglobulins of IgM class was determined in serum of peripheral blood of cows of the black-white breed, being after first, second and third lactation, in blood serum of calves in the period from birth to the 12th week of age as well as in beestings from the first milking. IgM level in serum and beestings was determined by redial immunodiffusion according to Mancini et al., as modified by Fahey and Mc Kelvey. Statistic analysis of the results obtained (double nonorthogonal crossing, correlation test) showed that after one, two or three lactations no changes in IgM level occurred in serum of cows in the last month of pregnancy and in beestings from the first milking. A relationship was found between IgM level in serum of pregnant cows, in beestings and in serum of calves fed with beestings at the age from the third day to one week.
测定了黑白花奶牛头胎、二胎和三胎产后外周血血清中IgM类免疫球蛋白水平，以及犊牛出生至12周龄期间血清中的IgM水平，还测定了首次挤奶的初乳中的IgM水平。血清和初乳中的IgM水平根据Mancini等人的方法，经Fahey和Mc Kelvey修改后，采用单向免疫扩散法测定。对所得结果进行统计分析（双非正交交叉、相关性检验）表明，头胎、二胎或三胎产后，怀孕最后一个月奶牛血清和首次挤奶的初乳中IgM水平无变化。发现怀孕奶牛血清、初乳以及在出生第三天至一周龄期间用初乳喂养的犊牛血清中的IgM水平之间存在关联。
