Uhlenbruck G, Janssen E, Javeri S
Immunobiology. 1982 Oct;163(1):36-47. doi: 10.1016/S0171-2985(82)80104-6.
Two different anti-galactan lectins from eel serum (Anguilla anguilla) were isolated by affinity chromatography on fucogel and on 1-acyl-2-(9-carboxy)nonyl-glycero-3-phosphocholine coupled to Aminohexyl-Sepharose. The fucolectin and the CRP-analogue protein thus purified directly from the eel serum were characterized by SDS-gradient gel electrophoresis, immunoelectrophoresis, and by agar gel diffusion tests. The CRP-analogue protein with a molecular weight of 24.800 Daltons was present in a high concentration. Carbohydrates are absent in both the proteins, showing that the fucolectin and the CRP analogue do not belong to the immunoglobulin/antibody group of serum proteins.
通过在岩藻糖凝胶和偶联到氨基己基琼脂糖上的1-酰基-2-（9-羧基）壬基甘油-3-磷酸胆碱上进行亲和层析，从鳗鱼血清（欧洲鳗鲡）中分离出两种不同的抗半乳聚糖凝集素。通过SDS梯度凝胶电泳、免疫电泳和琼脂凝胶扩散试验对由此直接从鳗鱼血清中纯化得到的岩藻糖凝集素和CRP类似蛋白进行了表征。分子量为24,800道尔顿的CRP类似蛋白以高浓度存在。两种蛋白质中均不存在碳水化合物，这表明岩藻糖凝集素和CRP类似物不属于血清蛋白的免疫球蛋白/抗体组。
