Søndergaard I
Allergy. 1982 Nov;37(8):581-6. doi: 10.1111/j.1398-9995.1982.tb02343.x.
A reversed phase ion-pair high performance liquid chromatographic (HPLC) method for quantitative determination of the major histamine metabolite 1,4-methyl-imidazoleacetic acid (1,4-MIAA) in urine is described. The sample handling is minimal as the sample only has to be centrifuged and diluted before analysis, in contrast to earlier gas chromatographic and thin-layer chromatographic methods. The method correlates well with a thin-layer chromatographic determination (r = 0.79, n = 15), and results from analysis of urine from different patients suffering from mastocytosis, chronic urticaria, atopic dermatitis and asthma are presented together with samples from normal individuals. Most of the patients analysed showed increased excretion of 1,4-MIAA compared with the controls. Future applications of the method, including analysis on patients with food allergy and patients with abnormalities in their histamine metabolism, are discussed.
描述了一种反相离子对高效液相色谱（HPLC）法，用于定量测定尿液中主要的组胺代谢物1,4-甲基咪唑乙酸（1,4-MIAA）。与早期的气相色谱法和薄层色谱法相比，样品处理非常简单，因为样品在分析前只需离心和稀释。该方法与薄层色谱测定法相关性良好（r = 0.79，n = 15），并给出了来自肥大细胞增多症、慢性荨麻疹、特应性皮炎和哮喘患者以及正常个体尿液分析的结果。与对照组相比，大多数分析的患者显示1,4-MIAA排泄增加。还讨论了该方法未来的应用，包括对食物过敏患者和组胺代谢异常患者的分析。
