Fujimoto S, Okui K
Gan To Kagaku Ryoho. 1982 Apr;9(4):582-9.
A sensitivity test of antitumor drugs using primary shortterm culture with 3H-thymidine was performed on stomach cancer patients. Stomach cancer tissues, which were removed in the operating room, were cut into cubes of 1 to 2 mm. Thereafter, they were put on a stainless steel mesh which was placed in a small petri dish (40 X 15 mm) and they were moisted with the cultivating medium. An appropriate dose of antitumor drugs (MM-C, 5-FU, cytosine arabinoside) was added at the onset of cultivation and 3H-thymidine was further added 24 hours later. At the end of cultivation in a CO2 incubator for about 3 days, the specimens were homogenized in an ice-cold homogenizer with 5% trichloroacetic acid. DNA fraction of the specimens was extracted by Schmidt-Thannhauser method. The isotope activity in DNA fraction was measured in a scintillation counter and was represented as cpm/microgram DNA. Sensitivities of antitumor drugs can be determined by comparison between isotope incorporations into the specimens tested and those into control specimens. Bacterial and fungal contaminations were observed in primary stomach cancer tissues.
对胃癌患者进行了使用含3H-胸腺嘧啶核苷的原代短期培养的抗肿瘤药物敏感性试验。将在手术室切除的胃癌组织切成1至2毫米的小块。之后，将它们放在置于小培养皿（40×15毫米）中的不锈钢网上，并用培养基湿润。在培养开始时加入适当剂量的抗肿瘤药物（丝裂霉素C、5-氟尿嘧啶、阿糖胞苷），24小时后再加入3H-胸腺嘧啶核苷。在二氧化碳培养箱中培养约3天后，将标本在冰冷的匀浆器中用5%三氯乙酸匀浆。用施密特-坦豪泽方法提取标本的DNA部分。在闪烁计数器中测量DNA部分的同位素活性，并表示为每微克DNA的计数每分钟（cpm）。通过比较测试标本和对照标本中同位素掺入情况可确定抗肿瘤药物的敏感性。在原发性胃癌组织中观察到细菌和真菌污染。
