Fensterbank R, Pardon P, Marly J
Ann Rech Vet. 1982;13(4):295-301.
In areas where the prevalence of Brucella melitensis infection in sheep is high, control of the disease cannot be led without the help of vaccination. The use of Brucella melitensis strain Rev. 1 vaccine affords a very good protection. The drawback is that this vaccine, giving rise to long-lasting antibody titers, may disturb serological screening when a return is made to a test and slaughter policy, after several years of vaccination. There is a great need for another mode of vaccination which would avoid this disadvantage. In this experiment, the potency of a vaccination by conjunctival route with 1.4 X 10(8) Rev. 1, plus a recall of 2.9 X 10(8) six months later, was compared with that of the normal subcutaneous vaccination with 1.4 or 2.7 X 10(9) Rev. 1. The vaccination by conjunctival route afforded a somewhat better protection than the subcutaneous one, and with a shorter serological response. It may be possible that a single conjunctival vaccination, avoiding the difficulties of vaccinating twice in ovine flocks and giving few antibodies, could also afford a good protection. Research in progress should include investigation of this point.
在绵羊布鲁氏菌感染率高的地区，没有疫苗接种的帮助就无法控制该病。使用布鲁氏菌Rev. 1疫苗可提供很好的保护。缺点是，这种疫苗会产生持久的抗体滴度，在经过数年疫苗接种后，当恢复到检测和屠宰政策时，可能会干扰血清学筛查。非常需要另一种可避免此缺点的疫苗接种方式。在本实验中，将结膜途径接种1.4×10⁸Rev. 1并在6个月后再接种2.9×10⁸的效力，与皮下接种1.4或2.7×10⁹Rev. 1的常规皮下接种效力进行了比较。结膜途径接种提供的保护比皮下接种略好，且血清学反应更短。单次结膜接种有可能避免在羊群中进行两次接种的困难且产生较少抗体，也能提供良好的保护。正在进行的研究应包括对此点的调查。
