Miller R R, Ayres J A, Rampy L W, McKenna M J
Fundam Appl Toxicol. 1981 Nov-Dec;1(6):410-4. doi: 10.1016/s0272-0590(81)80018-8.
Methyl, ethyl and butyl acrylate were hydrolyzed to acrylic acid in rat liver, kidney and lung homogenates. The rates of hydrolysis of the various esters in these in vitro studies were comparable; hydrolysis rates were approximately 20 times higher in liver homogenates than in kidney or lung homogenates. The esters also disappeared rapidly when added to blood in vitro. However, the disappearance in blood was not associated with the appearance of acrylic acid. Ethyl acrylate was found to react spontaneously with GSH in vitro and this reaction was catalyzed greatly by enzymes in 100 000 x g liver supernatant. Acrylic acid did not react with GSH in vitro. Ethyl acrylate, but not acrylic acid, depletes non-protein sulfhydryls when added to blood in vitro. Thus, the disappearance of acrylate esters in blood in vitro could be due at least in part to binding with non-protein sulfhydryls in red blood cells rather than to hydrolysis.
丙烯酸甲酯、丙烯酸乙酯和丙烯酸丁酯在大鼠肝脏、肾脏和肺组织匀浆中水解为丙烯酸。在这些体外研究中，各种酯的水解速率相当；肝脏匀浆中的水解速率比肾脏或肺组织匀浆中的水解速率高约20倍。当酯类在体外添加到血液中时，它们也会迅速消失。然而，血液中酯类的消失与丙烯酸的出现无关。发现丙烯酸乙酯在体外能与谷胱甘肽自发反应，并且该反应在100000×g肝脏上清液中的酶的极大催化下进行。丙烯酸在体外不与谷胱甘肽反应。当丙烯酸乙酯在体外添加到血液中时，会消耗非蛋白质巯基，而丙烯酸不会。因此，酯类在体外血液中的消失至少部分可能是由于与红细胞中的非蛋白质巯基结合，而不是由于水解。
