一种使用考马斯亮蓝G250对聚丙烯酰胺凝胶中的蛋白质进行染色的新技术。 - Suppr

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超能文献

A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250.

作者信息

Blakesley R W, Boezi J A

出版信息

Anal Biochem. 1977 Oct;82(2):580-2. doi: 10.1016/0003-2697(77)90197-x.

DOI:10.1016/0003-2697(77)90197-x

PMID:71866

Abstract

摘要

相似文献

A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue G250.一种使用考马斯亮蓝G250对聚丙烯酰胺凝胶中的蛋白质进行染色的新技术。

Anal Biochem. 1977 Oct;82(2):580-2. doi: 10.1016/0003-2697(77)90197-x.

Electrophoresis in the presence of Coomassie brilliant blue R-250 stains polyacrylamide gels during protein fractionation.在蛋白质分级分离过程中，考马斯亮蓝R - 250存在下的电泳可对聚丙烯酰胺凝胶进行染色。

Anal Biochem. 1984 Jul;140(1):84-6. doi: 10.1016/0003-2697(84)90136-2.

Quantitation of submicrogram amounts of protein using coomassie brilliant blue R on sodium dodecyl sulfate-polyacrylamide slab-gels.在十二烷基硫酸钠-聚丙烯酰胺平板凝胶上使用考马斯亮蓝R对亚微克量蛋白质进行定量分析。

Anal Biochem. 1975 Jul;67(1):347-52. doi: 10.1016/0003-2697(75)90305-x.

Application of Neuhoff's optimized Coomassie brilliant blue G-250/ammonium sulfate/phosphoric acid protein staining to ultrathin polyacrylamide gels on polyester films.Neuhoff优化的考马斯亮蓝G-250/硫酸铵/磷酸蛋白质染色法在聚酯薄膜上超薄聚丙烯酰胺凝胶中的应用。

Electrophoresis. 1988 May;9(5):236-8. doi: 10.1002/elps.1150090510.

Imidazole-SDS-Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins.在含有或不含十二烷基硫酸钠（SDS）的凝胶中对蛋白质进行咪唑-SDS-锌反向染色以及对单个未修饰的电转印蛋白质进行微测序

FEBS Lett. 1992 Jan 27;296(3):300-4. doi: 10.1016/0014-5793(92)80309-5.

Variations of Staining Sodium Dodecyl Sulfate-Polyacrylamide Gels with Coomassie Brilliant Blue.考马斯亮蓝染色 SDS-聚丙烯酰胺凝胶的变化。

Cold Spring Harb Protoc. 2021 Dec 1;2021(12):2021/12/pdb.prot102236. doi: 10.1101/pdb.prot102236.

Quantitation of proteins by elution of Coomassie brilliant blue R from stained bands after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后，通过从染色条带中洗脱考马斯亮蓝R来定量蛋白质。

Anal Biochem. 1986 May 15;155(1):23-7. doi: 10.1016/0003-2697(86)90218-6.

Fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counter ion-dyes, Coomassie brilliant blue R-250 and neutral red.使用抗衡离子染料、考马斯亮蓝R-250和中性红在十二烷基硫酸钠聚丙烯酰胺凝胶中进行快速蛋白质染色。

Arch Pharm Res. 2002 Oct;25(5):704-8. doi: 10.1007/BF02976948.

Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.使用考马斯亮蓝G-250和R-250在聚丙烯酰胺凝胶（包括等电聚焦凝胶）中实现蛋白质染色的改进，背景清晰，纳克级灵敏度。

Electrophoresis. 1988 Jun;9(6):255-62. doi: 10.1002/elps.1150090603.

Double staining of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected proteins.通过咪唑-十二烷基硫酸钠-锌反向染色对考马斯亮蓝染色的聚丙烯酰胺凝胶进行双重染色：灵敏检测考马斯亮蓝未检测到的蛋白质。

Anal Biochem. 1995 Jan 1;224(1):263-9. doi: 10.1006/abio.1995.1039.

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