Lee W H, Harris M E, McClain D, Smith R E, Johnston R W
Appl Environ Microbiol. 1980 Jan;39(1):205-9. doi: 10.1128/aem.39.1.205-209.1980.
Yersinia enterocolitica is one of the few human pathogens that grows at the proper food refrigeration temperatures of 0 to 5 degrees C. Although the isolation of environmental biotypes of Y. enterocolitica from many types of food and water has been reported in the literature, the recovery of the sensitive strains inoculated into foods has been slow and uncertain. Rapid recovery of several clinical strains inoculated into meats was accomplished by using two modified selenite broths without added nutrients. It was critical to restrict the sample size of the blended meat suspension at the 0.2-g/100 ml level, thereby restricting the growth of the total bacterial population in the selenite enrichment media. Otherwise, the slower growing Y. enterocolitica would be overwhelmed by the faster growing normal bacterial flora from the meat. Both the resistant serotype O:3 and the sensitive O:8 clinical isolates of Y. enterocolitica were recovered from the modified selenite enrichment media after 2 and 3 days of incubation at 22 degrees C.
小肠结肠炎耶尔森菌是少数能在0至5摄氏度适宜食品冷藏温度下生长的人类病原体之一。尽管文献中已报道从多种食品和水中分离出小肠结肠炎耶尔森菌的环境生物型，但接种到食品中的敏感菌株的回收率一直很低且不稳定。通过使用两种不添加营养成分的改良亚硒酸盐肉汤，成功实现了接种到肉类中的几种临床菌株的快速回收。将混合肉悬液的样本量限制在0.2克/100毫升水平至关重要，从而限制亚硒酸盐富集培养基中总细菌种群的生长。否则，生长较慢的小肠结肠炎耶尔森菌会被肉类中生长较快的正常细菌菌群所淹没。在22摄氏度下培养2天和3天后，从小肠结肠炎耶尔森菌的抗性血清型O:3和敏感O:8临床分离株中均从改良亚硒酸盐富集培养基中回收得到。
