从肉类中直接分离小肠结肠炎耶尔森菌和假结核耶尔森菌。
Direct isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis from meat.
作者信息
Fukushima H
出版信息
Appl Environ Microbiol. 1985 Sep;50(3):710-2. doi: 10.1128/aem.50.3.710-712.1985.
Studies were done to determine the usefulness of dilute alkali (KOH) treatment of meat samples for direct isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis, without enrichment. Virulent Y. enterocolitica and Y. pseudotuberculosis in pork contaminated with 10(2), 10(3), and 10(4) cells per g survived the direct KOH treatment and were never recovered by using KOH postenrichment treatment. From 6 (4.8%) of 125 samples of retail ground pork, four biotype 4 serotype O3 and one biotype 3B serotype O3 strains of Y. enterocolitica and one Y. pseudotuberculosis serotype 4b strain were recovered by using direct KOH treatment without enrichment. As these isolations were attained without using enrichment cultural procedures, they represent an important time-saving alternative to simplify and speed isolation of Yersinia spp. from meat.
开展了多项研究，以确定对肉类样本进行稀碱（氢氧化钾）处理用于直接分离小肠结肠炎耶尔森菌和假结核耶尔森菌（无需富集）的有效性。每克被10²、10³和10⁴个细胞污染的猪肉中的强毒小肠结肠炎耶尔森菌和假结核耶尔森菌在直接氢氧化钾处理后存活下来，且通过氢氧化钾富集后处理从未回收得到。在125份零售绞碎猪肉样本中，有6份（4.8%）通过直接氢氧化钾处理（无需富集）回收得到了4株生物型4血清型O3和1株生物型3B血清型O3的小肠结肠炎耶尔森菌菌株以及1株假结核耶尔森菌血清型4b菌株。由于这些分离是在不使用富集培养程序的情况下实现的，它们代表了一种重要的节省时间的替代方法，可简化并加速从肉类中分离耶尔森菌属。
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