Wolf H J, Christiansen M, Hanson R S
J Bacteriol. 1980 Mar;141(3):1340-9. doi: 10.1128/jb.141.3.1340-1349.1980.
The cellular structure of two yeast strains capable of growth on methane was investigated by electron microscopy. Microbodies were observed in cells of Sporobolomyces roseus strain Y and Rhodotorula glutinis strain CY when grown on methane but rarely when grown on glucose. The size of the microbodies and the number observed per cell in a thin section did not increase with culture age. No crystalline organization was observed within these organelles. Similar microbodies were also observed in cells of R. glutinis CY grown on hexadecane. The plasma membranes of both methane and hexadecane-grown cells exhibited increased invagination compared to that of glucose-grown cells. Catalase activity was detected in the microbodies of alkane-grown cells by using 3,3'-diaminobenzidine as a cytochemical stain. The data presented suggest that microbodies, and the catalase contained within them, play a role in eucaryotic methane metabolism.
通过电子显微镜对两种能够利用甲烷生长的酵母菌株的细胞结构进行了研究。当玫瑰掷孢酵母菌株Y和粘红酵母菌株CY在甲烷上生长时，在其细胞中观察到了微体，但在葡萄糖上生长时很少观察到。微体的大小以及在薄切片中每个细胞观察到的微体数量不会随着培养时间的延长而增加。在这些细胞器内未观察到晶体结构。在以十六烷为碳源生长的粘红酵母CY细胞中也观察到了类似的微体。与在葡萄糖上生长的细胞相比，以甲烷和十六烷为碳源生长的细胞的质膜内陷增加。通过使用3,3'-二氨基联苯胺作为细胞化学染色剂，在烷烃生长细胞的微体中检测到了过氧化氢酶活性。所呈现的数据表明，微体及其所含的过氧化氢酶在真核生物甲烷代谢中发挥作用。
