针对N-（鸟苷-8-基）-2-氨基芴的抗体。
Antibodies to N-(guanosin-8-yl)-2-aminofluorene.
作者信息
Rio P, Leng M
出版信息
Biochimie. 1980;62(7):487-90. doi: 10.1016/s0300-9084(80)80066-6.
PMID:7190850
Abstract
Antibodies to Guo-AF were elicited in rabbits by immunization with bovine serum albumin -Guo-AF conjugate. The antibodies were purified by affinity chromatography. The affinity of the antibodies towards several ligands was studied by radioimmunoassays. The antibodies recognize Gua-AAF and Guo-AF (K(Guo-AF)/K(Guo-AAF) congruent to 25). The circular dichroism of Guo-AF as a function of pH is reported.
摘要
通过用牛血清白蛋白 - 郭 - AF 偶联物免疫兔子，产生了针对郭 - AF 的抗体。这些抗体通过亲和色谱法进行纯化。通过放射免疫测定法研究了抗体对几种配体的亲和力。这些抗体识别瓜 - AAF 和郭 - AF（郭 - AF 的解离常数/瓜 - AAF 的解离常数约为 25）。报道了郭 - AF 的圆二色性随 pH 的变化情况。
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