Harris R C, Hultman E, Sahlin K
Pflugers Arch. 1981 Mar;389(3):277-82. doi: 10.1007/BF00584790.
Isometric contraction of the quadriceps muscle sustained to fatigue with a force of 66% of the maximum voluntary contraction force resulted in a mean glycogen utilization of 80.4 (S.D. 58.4) mmol glucosyl units/kg dry muscle (d.m.) and an accumulation of glycolytic intermediates and glucose corresponding to 82.9 (S.D. 17.5) mmol glucosyl units/kg d.m. Accumulation of hexose phosphates (principally glucose 6-phosphate) accounted for 35.4% (S.D. 4.1) of the total increase and lactate for 59.3% (S.D. 2.8). During a 4 min recovery period glucose 6-phosphate content showed a linear decrease with a half time of 2.0 min and lactate decreased exponentially with a half time of 2.5 min. The rate of lactate disappearance from the muscle was approximately 4 times as fast as that observed previously after maximal bicycle exercise. This was probably due to a lower lactate concentration in blood after isometric contraction resulting in a larger muscle-blood gradient for lactate. Muscle content of free glucose was increased after contraction and increased further during recovery. It is concluded that the glucose increase is confined to the intracellular pool and is an effect of hexokinase inhibition by accumulated glucose 6-phosphate. Occlusion of the local circulation after the contraction inhibited the recovery processes for lactate and glucose 6-phosphate.
股四头肌等长收缩持续至疲劳，收缩力为最大自主收缩力的66%，结果显示平均糖原利用率为80.4（标准差58.4）毫摩尔葡萄糖基单位/千克干肌肉（d.m.），糖酵解中间产物和葡萄糖的积累量相当于82.9（标准差17.5）毫摩尔葡萄糖基单位/千克d.m.。己糖磷酸（主要是6-磷酸葡萄糖）的积累占总增加量的35.4%（标准差4.1），乳酸占59.3%（标准差2.8）。在4分钟的恢复期内，6-磷酸葡萄糖含量呈线性下降，半衰期为2.0分钟，乳酸呈指数下降，半衰期为2.5分钟。肌肉中乳酸消失的速度大约是先前最大强度自行车运动后观察到的速度的4倍。这可能是由于等长收缩后血液中乳酸浓度较低，导致肌肉与血液之间的乳酸梯度更大。收缩后肌肉中游离葡萄糖含量增加，恢复期进一步增加。结论是葡萄糖的增加局限于细胞内池，是6-磷酸葡萄糖积累对己糖激酶抑制的结果。收缩后局部循环的阻断抑制了乳酸和6-磷酸葡萄糖的恢复过程。
