Yoshida T, Nagata A, Muro M, Takeuchi N, Suda Y
Eur J Appl Physiol Occup Physiol. 1981;46(4):423-30. doi: 10.1007/BF00422129.
Using an open circuit system (Douglas bag method), measurement of the anaerobic threshold (AT) was performed on ten healthy male college students during an incremental exercise test on a bicycle ergometer in an attempt to determine the validity of this method as compared with arterial blood lactate AT measurement. Blood samples were taken from either the radial or brachial artery through a Teflon catheter (3 ml/each time) every minute until the subject's maximal exercise tolerance was reached. Blood lactate was analyzed by the enzymatic method. Differences in work rate, VO2, %VO2max, VE, HR, and R at ATLA(AT determined by the increase in blood lactate) and at ATGE (gas exchange AT based on the non-linear increases in VE, VCO2, and other respiratory parameters), respectively, were all found to be statistically insignificant. There was a significant correlation (r = 0.866, p less than 0.01) between ATLA and ATGE when expressed i VO2 values (l/min). There was also a significant correlation between ATLA and VO2max (r = 0.778, p less than 0.01). These results indicate that the commonly used Douglas bag method could provide a valid non-invasive measure of anaerobic threshold.
采用开路系统（道格拉斯袋法），在自行车测力计递增运动试验期间，对10名健康男性大学生进行无氧阈（AT）测量，以确定该方法与动脉血乳酸AT测量相比的有效性。通过聚四氟乙烯导管从桡动脉或肱动脉每隔一分钟采集血样（每次3毫升），直至达到受试者的最大运动耐力。采用酶法分析血乳酸。分别在基于血乳酸增加确定的ATLA（无氧阈）和基于VE、VCO2及其他呼吸参数非线性增加的ATGE（气体交换无氧阈）时，工作率、VO2、%VO2max、VE、HR和R的差异均无统计学意义。以VO2值（升/分钟）表示时，ATLA和ATGE之间存在显著相关性（r = 0.866，p < 0.01）。ATLA和VO2max之间也存在显著相关性（r = 0.778，p < 0.01）。这些结果表明，常用的道格拉斯袋法可提供有效的无创无氧阈测量方法。
