van Leeuwen F W, Swaab D F, de Raay C
Cell Tissue Res. 1978 Oct 6;193(1):1-10. doi: 10.1007/BF00221596.
The classical areas for arginine-vasopressin (AVP) synthesis are the magnocellular supraoptic (SON) and paraventricular nuclei. More recently AVP was also demonstrated in neurons of the parvocellular suprachiasmatic nucleus (SCN) of the rat. This result was substantiated in the present study by means of immunoelectron microscopy, by subjecting sections to antivasopressin plasma. Conventional electron microscopy revealed dense-core vesicles in the SCN cell bodies and fibres (mean diameter 94.7 +/- 0.9 nm and 84.0 +/- 1.1 nm respectively). These vesicles were infrequent within the cell bodies and could not be accumulated by ethanol administration. Immunoelectron microscopy showed a positive reaction in the cell bodies and fibres within vesicles of 96.7 +/- 1.1 nm and 98.5 +/- 1.1 nm and 98.5 +/- 1.2 nm respectively. By comparison, the cell bodies and fibres of the SON showed immunoreactive granules of 143.0 +/- 1.8 and 147.3 +/- 1.8 nm respectively. The presence in the SCN of AVP in vesicles of different size than those in the SON suggests that synthesis of this substance is indeed occurring within the SCN cells.
精氨酸加压素（AVP）合成的经典区域是视上核（SON）和室旁核的大细胞。最近，在大鼠的视交叉上核（SCN）的小细胞神经元中也发现了AVP。本研究通过免疫电子显微镜，用抗加压素血浆处理切片，证实了这一结果。传统电子显微镜显示，SCN细胞体和纤维中有致密核心囊泡（平均直径分别为94.7±0.9nm和84.0±1.1nm）。这些囊泡在细胞体内很少见，且不能通过给予乙醇而积累。免疫电子显微镜显示，细胞体和纤维内的囊泡分别在直径为96.7±1.1nm、98.5±1.1nm和98.5±1.2nm的囊泡中有阳性反应。相比之下，SON的细胞体和纤维分别显示出直径为143.0±1.8nm和147.3±1.8nm的免疫反应性颗粒。SCN中存在的AVP囊泡大小与SON中的不同，这表明该物质确实在SCN细胞内合成。
