Satoh K
Clin Chim Acta. 1978 Nov 15;90(1):37-43. doi: 10.1016/0009-8981(78)90081-5.
A new colorimetric method for quantitative analysis of serum lipid peroxide, free of interference from sialic acids, has been developed. We have used the thiobarbituric acid dissolved in sodium sulfate solution and both liberation of lipid peroxide and color reaction have been performed simultaneously by heating serum protein precipitate with this reagent in a weak acid solution. The new method is specific and facilitates the precise measurements of serum lipid peroxide. The average values determined by the new method increased slightly with age in healthy subjects. In patients with sequelae of cerebrovascular disorders, serum lipid peroxide values were higher than in healthy controls. These results may demonstrate the important role of lipid peroxide in aging and cerebrovascular disorders.
已开发出一种用于血清脂质过氧化物定量分析的新比色法，该方法不受唾液酸干扰。我们使用溶解在硫酸钠溶液中的硫代巴比妥酸，通过在弱酸溶液中用该试剂加热血清蛋白沉淀，同时进行脂质过氧化物的释放和显色反应。新方法具有特异性，便于精确测量血清脂质过氧化物。在健康受试者中，用新方法测定的平均值随年龄略有增加。在脑血管疾病后遗症患者中，血清脂质过氧化物值高于健康对照组。这些结果可能证明脂质过氧化物在衰老和脑血管疾病中的重要作用。
