甲状旁腺激素敏感细胞化学测定法的初步研究。

Preliminary studies with the sensitive cytochemical assay for parathyroid hormone.

作者信息

Fenton S, Somers S, Heath D A

出版信息

Clin Endocrinol (Oxf). 1978 Oct;9(4):381-4. doi: 10.1111/j.1365-2265.1978.tb02224.x.

DOI:10.1111/j.1365-2265.1978.tb02224.x

PMID:719908

Abstract

A modification of the cytochemical assay for parathyroid hormone (PTH) is described which permits the measurement of as little as 0.1 fg ml-1. Using this assay circulating concentrations in normal humans range between 1 and 30 pg ml-1 using a bovine PTH standard. Elevated values are found in hyperparathyroidism with reduced concentrations in hypoparathyroidism. Where detectable, PTH concentrations measured by radioimmunoassay are on average fourteen-fold higher than by cytochemical assay. No cross reactivity was found with AVP, calcitonin and 1,25 dihydroxy vitamin D in concentrations normally found in health or disease.

摘要

本文描述了一种甲状旁腺激素（PTH）细胞化学检测方法的改进，该方法能够检测低至0.1 fg/ml-1的PTH。使用该检测方法，以牛PTH标准品测定，正常人体内循环浓度范围为1至30 pg/ml-1。甲状旁腺功能亢进时PTH值升高，甲状旁腺功能减退时浓度降低。在可检测的情况下，放射免疫法测得的PTH浓度平均比细胞化学检测法高14倍。在健康或疾病状态下正常出现的浓度范围内，未发现与抗利尿激素（AVP）、降钙素和1,25-二羟维生素D有交叉反应。