Kiehlbauch J A, Brenner D J, Cameron D N, Steigerwalt A G, Makowski J M, Baker C N, Patton C M, Wachsmuth I K
Foodborne and Diarrheal Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1995 Nov;33(11):2940-7. doi: 10.1128/jcm.33.11.2940-2947.1995.
By DNA-DNA hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as Helicobacter cinaedi as well as 2 human strains and 2 animal strains of Helicobacter fennelliae. All but one human strain belonged to the same hybridization group as the type strain of H. cinaedi. The animal strains also appeared to belong to this hybridization group. Both human strains of H. fennelliae were shown to be H. fennelliae by DNA-DNA hybridization, but both animal strains were less than 15% related to the type strain. All strains were also characterized by plasmid profiles and ribotyping. Plasmids were found in 23% of the human strains, 100% of the hamster strains, and 33% of the dog and cat strains. Human strains were essentially identical by ribotyping, but were clearly differentiated from the hamster and dog and cat strains. Some strains may be difficult to culture on primary isolation; we found that our strains grew well on anaerobic CDC agar, brucella agar, and tryptic soy agar II. Our H. cinaedi and H. fennelliae strains differed from those previously described because some were resistant to cephalothin: some H. cinaedi strains were also resistant to nalidixic acid. All isolates were also characterized by antimicrobial susceptibility testing. We found that human strains of H. cinaedi were more resistant to clindamycin and erythromycin than were animal isolates; 19% of the human strains were resistant to ciprofloxacin. Therefore, we recommend that antimicrobial susceptibility results be obtained before initiating therapy for H. cinaedi and H. fennelliae infections.
通过DNA-DNA杂交，我们对26株人源菌株、4株犬猫源菌株和4株仓鼠源菌株进行了分类，这些菌株被初步鉴定为犬西螺杆菌，同时还对2株人源芬内尔螺杆菌菌株和2株动物源芬内尔螺杆菌菌株进行了分类。除1株人源菌株外，所有菌株均与犬西螺杆菌模式菌株属于同一杂交组。动物源菌株似乎也属于该杂交组。两株人源芬内尔螺杆菌菌株经DNA-DNA杂交显示为芬内尔螺杆菌，但两株动物源菌株与模式菌株的相关性均低于15%。所有菌株还通过质粒图谱和核糖体分型进行了特征分析。在23%的人源菌株、100%的仓鼠源菌株和33%的犬猫源菌株中发现了质粒。人源菌株的核糖体分型基本相同，但与仓鼠源以及犬猫源菌株明显不同。一些菌株在初次分离时可能难以培养；我们发现我们的菌株在厌氧的CDC琼脂、布鲁氏菌琼脂和胰蛋白胨大豆琼脂II上生长良好。我们的犬西螺杆菌和芬内尔螺杆菌菌株与先前描述的菌株不同，因为有些对头孢噻吩耐药：一些犬西螺杆菌菌株还对萘啶酸耐药。所有分离株还通过抗菌药物敏感性试验进行了特征分析。我们发现，人源犬西螺杆菌菌株比动物源分离株对克林霉素和红霉素的耐药性更强；19%的人源菌株对环丙沙星耐药。因此，我们建议在开始治疗犬西螺杆菌和芬内尔螺杆菌感染之前获得抗菌药物敏感性结果。
